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Figure 2 | Molecular Neurodegeneration

Figure 2

From: Prion subcellular fractionation reveals infectivity spectrum, with a high titre-low PrPreslevel disparity

Figure 2

PrPres is enriched in lipid raft fractions of MoRK13-inf cells. (A) Equivalent volumes of PK treated MoRK13-inf fractions resolved on 10-20% Tris-glycine SDS PAGE and immunoblotted (top panel), and representative fraction PrPres dot blot (bottom panel). (B) Quantification of PrPres from dot blots of six separate cellular fractionations. Fractions enriched in lipid raft (LR), endoplasmic reticulum (ER), mitochondrial (MT) and early endosome (EE) marker proteins are highlighted. Comparison of relative PrPres levels by one way ANOVA; *** p < 0.001, ns = not significant.

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Molecular Neurodegeneration

ISSN: 1750-1326