Figure 7
Sonication but not PK treatment alters efficiency of in vitro infection with fraction #4 only. (A) Representative cell blot (left panel) of PrPres produced by MoRK13 cells exposed to selected MoRK13-inf fractions which were PK digested (PK) or left untreated (UN) and western blot (right panel) of MoRK13-inf inocula before and after PK treatment. (B) Quantification of cell blot PrPres levels (n = 3 independent experiments), with PrPres produced after infection with PK treated inocula expressed relative to untreated MoRK13-inf fraction inocula. (C) Representative cell blot (left panel) of PrPres produced by MoRK13 cells exposed to selected MoRK13-inf fractions which were sonicated (SON) or left untreated (UN) and western blot (right panel) of MoRK13-inf fraction inocula before and after sonication. (D) Quantification of cell blot PrPres levels expressed relative to untreated MoRK13-inf fraction inocula (n = 3 independent experiments). Note, levels of total PrP in fractions #8 and #10 are at or below the limits of detection by standard immunoblotting. Comparison of relative PrPres levels by one way ANOVA and Dunnett's post-test for specific comparisons to a control (ie untreated fraction) found no significant differences in any PK treated inocula, and only sonicated MoRK13-inf fraction #4 was significantly different compared to its untreated control, *p < 0.05.