Loss of LRRK2 causes age-dependent bi-phasic alterations of protein carbonyls. There is no significant difference in the levels of protein carbonyls, determined using OxyBlots, in both RIPA buffer-soluble and -insoluble fractions of kidneys between LRRK2-/- mice and wild-type (+/+) controls at one month of age. But the level of protein carbonyls is significantly reduced in the RIPA buffer-insoluble fractions of LRRK2-/- kidneys at 7 months of age compared with wild-type controls. By 20 months of age, the level of protein carbonyls is significantly increased in both RIPA buffer-soluble and -insoluble fractions of LRRK2-/- kidneys. ns, not significant; **, P < 0.01.