Figure 2

Comparison of the embolic versus filament ischemia models. a rCBF index before and during ischemia, as well as after reperfusion. There was no significant difference in rCBF in two-hour ischemia between mice with either type of ischemia. rCBF showed a 75% reduction relative to pre-ischemic baselines in 79% (68 out of 86) of the animals with embolic ischemia, comparable to the previous reports [29, 34–36]. b Behavioral tests of motor, sensory and reflex function scored using the mNSS on a scale from 0 to 14 (0 = normal, 14 = maximal deficit). Analysis indicated no statistically significant difference between embolic and filament models; p > 0.05, one-tailed Student’s t-test for the sum of behavioral scores. c Infarct volume quantified by 2,3,5-triphenyl-tetrazolium chloride (TTC) staining. No statistically significant difference was seen in infarct volumes in the two models; p > 0.05, by one-tailed Student’s t-test. Number of animals (n) in each group is shown in parenthesis, and data are expressed as means ± SEM. These results indicate that both focal cerebral ischemia models are comparable.