Figure 3

SB-3CT protects against brain damage and ameliorates neurobehavioral deficits after embolic MCA occlusion in mice. Mice were injected ip with SB-3CT (25 mg/kg body weight) or vehicle 2 and 4 hours after embolus-induced focal cerebral ischemia. Neurobehavioral tests were conducted 24 hours later and scored using the 14-point mNSS, followed by assessment of infarct volume by TTC staining. a Representative images of TTC staining. b Quantification of infarct volumes analyzed by TTC staining using ImageJ tools. *, p < 0.05 by one-tailed Student’s t-test. c Improvement in motor and sensory function following post-ischemic SB-3CT treatment compared to the vehicle-treated controls; *, p < 0.05 for motor; ***, p < 0.001 for sensory; and **, p < 0.01 for the sum of the behavioral scores, respectively, by one-tailed Student’s t-test. Number of animals (n) in each group is shown in parenthesis, and data are expressed as means ± SEM, whereas reflex activity was unchanged.