Figure 4
From: Regulation of mitochondrial permeability transition pore by PINK1
Effects of mPTP agonists and inhibitors on its opening in PINK1 −/− cells. A. Representative flow cytometry dot plots showing calcein fluorescence in PINK1−/− and +/+ cells treated with calcein-AM (1 μM) and Co2+ (1 mM) under basal conditions or following various treatment, such as in presence of the mPTP agonist atractylate (20 μM), the mPTP inhibitors Cyclosporine A (CsA, 1 μM) or Bongkrekic acid (BkA, 10 μM), or the calcineurin inhibitor FK-506 (5 μM). B. The bar graph shows quantification of calcein fluorescence after FACS analysis following various treatment, such as atractylate (20 μM), CsA (1 μM), BkA (10 μM), or FK-506 (5 μM). The number shown in the panel indicates the number of independent experiments performed. C. Representative images showing calcein fluorescence in PINK1−/− and +/+ cortical neurons incubated with calcein-AM (1 μM) and Co2+ (1 mM) under basal conditions or in the presence of atractylate (20 μM), CsA (1 μM), BkA (10 μM) or FK-506 (5 μM). D. Quantification of calcein fluorescence in PINK1−/− and +/+ neurons following various treatment with atractylate (20 μM), CsA (1 μM) or BkA (10 μM) or FK-506 (5 μM). The numbers shown in panels indicate the number of cells used (left) and the number of independent experiments performed (right) in the study. All data are expressed as mean ± SEM. * p < 0.05.