SORT1 triggers endocytosis of rPGRN. (A) Western blot analysis of Neuro2A cells transduced with rAAV-GFP or rAAV-SORT1 in the presense of His-rPGRN or rPGRN-His. Note uptake of His-rPGRN into cell lysates and the absence of rPGRN-His uptake. (B) Bar graph quantification of WT primary neuronal cultures treated with increasing 25, 125, 625 nM doses of His-rPGRN or rPGRN-His depict equal amounts of outgrowth with between treatments (nM doses correspond to 2, 10, 50 ug/mL concentrations respectively). Data is presented as the mean ± SEM.