Figure 6

Differential effect of cytokines on Aβ40 and fAβ42 uptake by primary mouse glia and astrocytes. A-B. mIL-4 treatment decreases microglial uptake of Aβ40 (A) but does not affect fAβ42 uptake (B). mIL-6 increased microglial Aβ40 uptake (A) but does not significantly alter fAβ42 uptake (B). Primary mouse glia were treated with recombinant cytokines for 10 hrs and incubated with Aβ40-Hilyte555 or Aβ42-Hilyte555 for 15 min, 30 min and 60 min. Trypsinized cells were counted using Accuri6 flow cytometer. Unstained cells and labeled additives were excluded by gating to yield the percentage of fluorescent cells in the mix. Inset depicts cd11b/DAPI stained glia (A) Results are representative of three independent experiments. (*p < 0.05, One way Anova with Tukey’s post test). Data represents mean ± sem. C-D. mIL-4 treatment does not affect astrocytic uptake of Aβ40 (C) but increases fAβ42 uptake after 60 min incubation (D). mIL-6 consistently increases astrocytic Aβ40 uptake (C) and only enhances fAβ42 uptake after 60 min incubation (D). Primary mouse astrocytes were treated with recombinant cytokines for 10 hrs and incubated in Aβ40-Hilyte555 or Aβ42-Hilyte555 for 15 min, 30 min and 60 min. Trypsinized cells were counted using Accuri6 flow cytometer. Unstained cells and labeled additives were excluded by gating to yield the percentage of fluorescent cells. Inset depicts GFAP/DAPI labeled astrocyte (C). Results are representative of two independent experiments. (*p < 0.05, One way Anova with Tukey’s post test). Data represents mean ± sem.