Figure 7

NAD⁺ does not protect dopaminergic neurons through the Sirt1 pathway. (A) Dissociated midbrain cultures from both WT and Sirt1 KO mice were pretreated with NAD⁺ 24 hours before addition of 2 μm MPP⁺. (B) Quantification of TH+ cell bodies and (C) TH+ neurites show that NAD⁺ protects cells and neurites from MPP⁺ in both WT and Sirt1 KO cultures. Data are normalized to control cultures and denote the mean ± SEM of representative determinations made in three separate cultures. *p < 0.05.