Figure 1

C. elegans Aß model accumulates full length Aß _{1– 42} . A ESI-MS analysis of immuno-precipitated material from C. elegans expressing Aß_1-42. Insert shows an observed species of mono-isotopic molecular weight 4511.2586 (2.5 ppm error) and Aß_1-42 plus a single oxygen (observed mass 4527.2710). Expected mass of Aß_1-42 is 4511.2697 Da. B TBS soluble lysate from C. elegans and synthetic Aβ species resolved on the basis of hydrophobicity and immuno-detected using 4G8 as the primary antibody. Synthetic standards consist of Aβ_1-42 and Aβ_3-42. Included are (4-day-old) transgenic control strain CL2122, Aβ_3-42 expressing CL2120, and Aβ_1-42 expressing GMC101. C Total protein extracts from C. elegans resolved via Tricine-SDS-PAGE with immuno-detection of Aß (using 6E10) and α-tubulin. D Plot of average total Aß normalized against α-tubulin, shown are means ± SD from n = 3 replicates.