Figure 1
From: Intraneuronal Aβ detection in 5xFAD mice by a new Aβ-specific antibody
MOAB-2 detects multiple Aβ conformations at low antibody concentrations. (A) Dot blot of serial Aβ40 (unaggregated) and Aβ42 dilutions (unaggregated (U), oligomeric (O) or fibrillar (F)) probed with MOAB-2 or 6E10. (B) Antibody-binding saturation curve of 25 ng Aβ42 in the different aggregation states (U, O, and F) immobilized on microtiter plates, incubated with different MOAB-2 concentrations as indicated on figure. (C) Western-blot analysis of 1000 pmol U-Aβ40 or 200 pmol U-Aβ42 probed with MOAB-2 (lanes 1-2) or 200 pmol U-Aβ40 and U-Aβ42 probed with 6E10(lanes 3-4). (D) Western-blot analysis comparing antibody dilutions of MOAB-2 and 6E10 (0.5 mg/ml antibody stock concentrations) using U-, O- and F-Aβ42 (200 pmol). Quantification of Aβ immunoreactivity for each antibody dilution estimated using image J. (E) Immunoprecipitation of U- and O-Aβ42 (0.5 pmol) with 6E10 or MOAB-2 (Lane 1 = IP-6E10, 2 = IP-MOAB-2, 3 = 5% total input (0.025 pmol), 4 = 10% total input (0.05 pmol). The dilution of MOAB-2 for Figures 1A and C is 1:5000 from a stock of 0.5 mg/ml.