Figure 5
From: FE65 as a link between VLDLR and APP to regulate their trafficking and processing
FE65 and VLDLR CTF complex translocates into the nucleus. A. COS7 cells were transfected with APP CTF and vector or APP CTF and FE65 and cellular fractionation was conducted. Cytoplasmic/Membrane (CM) and nuclear (N) fractions were immunoblotted with the C1/6.1 antibody (for APP) and the anti-myc antibody (for FE65). B. COS7 cells were transfected with full length VLDLR and vector or full length VLDLR and FE65 and cellular fractionation was conducted. Cytoplasmic/Membrane (CM) and nuclear (N) fractions were immunoblotted with the 5F3 antibody (for VLDLR) and the anti-myc antibody (for FE65). C. COS7 cells were transfected with VLDLR CTF and vector or VLDLR CTF and FE65 and cellular fractionation was conducted. Cytoplasmic/Membrane (CM) and nuclear (N) fractions were immunoblotted with the anti-HA antibody (for VLDLR CTF) and the anti-myc antibody (for FE65).