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Volume 7 Supplement 1

Proceedings of the 2011 International Conference on Molecular Neurodegeneration

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Protective effect of uric acid against 6-OHDA-induced injury in SH-SY5Y cells

Molecular Neurodegeneration volume 7, Article number: S12 (2012) Cite this article

Objective

To investigate the effect of uric acid (UA) on 6-hydroxydopamine (6-OHDA) -induced injury in SH-SY5Y cells.

Methods

The cell viability was measured by the MTT reduction assay. The cell apoptosis was assessed by Hoechst 33342 staining with fluorescence microscopy. The phophorylation of Akt and GSK-3β(ser9) was determined by Western blot analysis.

Results

Treatment with 6-OHDA at 50µM for 12 h significantly decreased the viability of SH-SY5Y cells. Pretreatment with UA (200-400µM, 0.5h) prior to 6-OHDA treatment markedly increased the cell viability of SH-SY5Y cells, as compared to that of 6-OHDA-treated group. The beneficial effects of UA against 6-OHDA-induced apoptosis were also confirmed by Hoechst 33342 staining assay. Moreover, 6-OHDA decreased the Akt activity and increased the GSK-3β activity, which could be blocked by UA (200-400µM) pretreatment.

Conclusions

These data suggest that 6-OHDA-induced cell injury was attenuated by UA. The underlying mechanisms may involve the up-regulation of Akt and the reduction of GSK-3β activity.

Acknowledgements

Contract grant sponsor: The work was supported by the Suzhou Foundation for Development of Science, Technology (200815404), Jiangsu Ordinary University Science Research Project (08KJB320012) and Natural Science Foundation of Jiangsu Province, China (BK2010229).

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Authors and Affiliations

  1. The Department of Neurology, Second Affiliated Hospital of Soochow University, Suzhou, China

    Tingting Huang, Li Gong, Weifeng Luo & Chunfeng Liu

  2. The Institute of Neuroscience of Soochow University, Suzhou, China

    Tingting Huang, Li Gong, Weifeng Luo, Lifang Hu & Chunfeng Liu

Authors
  1. Tingting Huang
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  2. Li Gong
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  3. Weifeng Luo
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  4. Lifang Hu
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  5. Chunfeng Liu
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Corresponding author

Correspondence to Weifeng Luo.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Huang, T., Gong, L., Luo, W. et al. Protective effect of uric acid against 6-OHDA-induced injury in SH-SY5Y cells. Mol Neurodegeneration 7 (Suppl 1), S12 (2012). https://doi.org/10.1186/1750-1326-7-S1-S12

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  • DOI: https://doi.org/10.1186/1750-1326-7-S1-S12

Keywords

Molecular Neurodegeneration

ISSN: 1750-1326