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Protective effect of uric acid against 6-OHDA-induced injury in SH-SY5Y cells


To investigate the effect of uric acid (UA) on 6-hydroxydopamine (6-OHDA) -induced injury in SH-SY5Y cells.


The cell viability was measured by the MTT reduction assay. The cell apoptosis was assessed by Hoechst 33342 staining with fluorescence microscopy. The phophorylation of Akt and GSK-3β(ser9) was determined by Western blot analysis.


Treatment with 6-OHDA at 50µM for 12 h significantly decreased the viability of SH-SY5Y cells. Pretreatment with UA (200-400µM, 0.5h) prior to 6-OHDA treatment markedly increased the cell viability of SH-SY5Y cells, as compared to that of 6-OHDA-treated group. The beneficial effects of UA against 6-OHDA-induced apoptosis were also confirmed by Hoechst 33342 staining assay. Moreover, 6-OHDA decreased the Akt activity and increased the GSK-3β activity, which could be blocked by UA (200-400µM) pretreatment.


These data suggest that 6-OHDA-induced cell injury was attenuated by UA. The underlying mechanisms may involve the up-regulation of Akt and the reduction of GSK-3β activity.


Contract grant sponsor: The work was supported by the Suzhou Foundation for Development of Science, Technology (200815404), Jiangsu Ordinary University Science Research Project (08KJB320012) and Natural Science Foundation of Jiangsu Province, China (BK2010229).

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Correspondence to Weifeng Luo.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Huang, T., Gong, L., Luo, W. et al. Protective effect of uric acid against 6-OHDA-induced injury in SH-SY5Y cells. Mol Neurodegeneration 7 (Suppl 1), S12 (2012).

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