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The role of ATP-sensitive potassium channels on DMT1-mediated ferrous iron uptake in SK-N-SH cells

Background

Elevated iron accumulation has been reported in the substantia nigra (SN) in Parkinson’s disease (PD). Our previous study observed that increased levels of the iron importer divalent metal transporter 1 (DMT1) were involved in this nigral iron accumulation and dopaminergic neurons loss in PD. The iron transport function of DMT1 is also related to the membrane potential level, which increases with hyperpolarization of the cell membrane. Activation of ATP-sensitive potassium (KATP) channels, which could induce hyperpolarization of nigral dopaminergic neurons, is reported to be involved in the selective loss of these neurons in PD. The present study is to investigate whether activation of KATP channels could change the iron uptake function of DMT1.

Methods

DiBAC4(3) and calcein were used to detect membrane potential and ferrous iron influx. Intracellular iron concentration was measured using an inductively coupled plasma (ICP-2) detector. Mitochondrial transmembrane potential (ΔΨm) and reactive oxygen species (ROS) were measured by flow cytometry using rhodamine123 and H2DCF-DA.

Results

(1) When treated with diazoxide, a novel KATP channel opener, the membrane potential of SK-N-SH cells showed hyperpolarization. (2) The influx of ferrous iron and the intracellular iron levels were observed dramatically increased when the cells were co-incubated with diazoxide and ferrous iron, resulting in a decreased ΔΨm and an elevated level of ROS production. (3) When treated with diazoxide, cells with DMT1 knockdown showed decreased ferrous iron influx compared with the vector control. (4) When treated with diazoxide, cells overexpressed of SUR1 and Kir6.2 showed increased ferrous iron influx compared with the vector control, which induced a decrease in ΔΨm and an increase in ROS production.

Conclusion

These results suggest that the activation of the KATP channels could enhance DMT1-mediated ferrous iron uptake, leading to increase intracellular oxidative stress.

Acknowledgements

This work was supported by grants from the National Program of Basic Research sponsored by the Ministry of Science and Technology of China (2011CB504100), the National Foundation of Natural Science of China (30930036) and the Natural Science Fund of Shandong Province for Distinguished Young Scholars (JQ200807).

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Correspondence to Junxia Xie.

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Du, X., Xu, H., Song, N. et al. The role of ATP-sensitive potassium channels on DMT1-mediated ferrous iron uptake in SK-N-SH cells. Mol Neurodegeneration 7, S6 (2012). https://doi.org/10.1186/1750-1326-7-S1-S6

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Keywords

  • Reactive Oxygen Species Production
  • Ferrous Iron
  • KATP Channel
  • Diazoxide
  • Iron Accumulation