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Figure 4 | Molecular Neurodegeneration

Figure 4

From: Widespread aggregation of mutant VAPB associated with ALS does not cause motor neuron degeneration or modulate mutant SOD1 aggregation and toxicity in mice

Figure 4

No significant pathological change in 18-month old VAPB transgenic mice. Spinal cord sections (A) of the ventral horn area and L3 ventral root nerve sections (B) from different genotypes are shown. All plastic sections were cut at 1 μm thickness and stained with toluidine blue. Scale bars: 20 μm. (C) Numbers of total axons of the ventral roots (L3 and L4) from 18-month old mice were counted. No significant difference in the numbers of axons was found between muVAPB35 (n = 5) and NTG (n = 4) mice. All values are shown as averages with standard deviation. (D) No evidence of microgliosis and astrogliosis was detected in the spinal cord of muVAPB35/60 mice, as compared with NTG mice. The sections from the lumbar spinal cords were stained with Iba1 and GFAP antibodies (green fluorescence) to show microglia and astrocytes, respectively. The nuclei were shown by DAPI stain. Scale bars: 20 μm. (E) No pathological change in the muscles from transgenic mice. Gastrocnemius muscle sections from NTG, wtVAPB, and mutVAPB35/60 were stained with H&E. Scale bars: 50 μm.

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Molecular Neurodegeneration

ISSN: 1750-1326