Figure 1
From: In vivo measurement of apolipoprotein E from the brain interstitial fluid using microdialysis
Analysis of apoE levels by microdialysis in vitro and in vivo . A. Microdialysis samples were collected hourly from human CSF in vitro at flow rates ranging from 0.4 μL/min to 1.6 μL/min. The concentration of apoE within microdialysis samples and CSF was determined by ELISA. Data points represent mean ± SEM (n=3). A single-phase exponential decay curve (r2=0.93) was used to calculate the estimated mean concentration of apoE at zero flow. B. Microdialysis probes were implanted into the hippocampus of 3-4 month old mice and ISF collected bi-hourly for 36 hours. The concentration of apoE within microdialysis samples was determined by ELISA. Data points represent mean ± SEM (n=5). C. The mean concentration of murine apoE in ISF microdialysis samples from WT (n=5) and apoE KO mice (n=2) was determined by ELISA. ISF samples were collected at a constant flow rate of 1.0 μL/min. Data are presented as mean ± SEM.