Figure 3

The levels of the mitochondrial proteins Tom40 and COX1 in hippocampal neurons of 4-month-old apoE3 and apoE4 mice. (A) Tom40 immunohistochemistry. Representative images (X10 magnification) of the indicated hippocampal subfields are presented on the left. Quantification of the results (mean ± SEM; n=8 per group) of apoE3 mice (white bars) and apoE4 mice (black bars) is shown on the right. *p<0.05. Scale = 350 μ. (B) COX1 immunohistochemistry. Representative images (X10 magnification) of the indicated hippocampal subfields are presented on the left. Quantification of the results (mean ± SEM; n=8 per group) of apoE3 mice (white bars) and apoE4 mice (black bars) is shown on the right. *p<0.05. Scale = 350 μ. Quantification of the Tom40 and COX1 results was performed by computerized image analysis, as described in Materials and Methods. (C) Representative images of CA3 at X100 magnification are shown. Scale = 30 μ. Inserts depict selected neurons at X2 magnification. (D) Tom40 and COX1 immunoblots. Representative immunoblots of homogenates of hippocampal CA3 neurons of apoE3 and apoE4 mice are presented on the left together with the GAPDH standard. Quantification of the results (mean ± SEM; n=5 per group) of apoE3 mice (white bars) and apoE4 mice (black bars) is depicted on the right. *p<0.05.