Figure 4

The levels of Aβ42 in hippocampal neurons of 4-month-old apoE3 and apoE4 mice. (A) Representative images (X10 magnification) of the indicated hippocampal fields stained with anti-Aβ42 are presented on the left. Quantification of the results (mean ± SEM; n=20 per group) of apoE3 mice (white bars) and apoE4 mice (black bars) was performed by computerized image analysis, as described in Materials and Methods, and is shown on the right. *p<0.05. Scale = 350 μ. (B) Representative images of CA3 at X100 magnification are shown. Scale = 30 μ. Inserts depict selected neurons at X2 magnification. (C) Representative confocal images (X20 magnification) of the CA3 subfield of apoE3 and apoE4 mice stained with the pan anti-Aβ Ab 4G8. Scale = 120 μ. (D) Aβ X-42 ELISA. Quantification of Aβ42 levels in 4-month-old apoE3 and apoE4 mice was performed utilizing an ELISA kit. Results are presented as pictograms of Aβ42 per hippocampus. *p<0.05