Figure 7

The levels and spatial distribution of apoE in the hippocampus of 1- and 4-month-old apoE3 and apoE4 mice. (A) ApoE immunoblots. Representative immunoblots of homogenates of whole hippocampi of 1- and 4-month-old apoE3 and apoE4 mice are presented on the left together with the GAPDH standard. Quantification of the results (mean ± SEM; n=6 per group) of apoE3 mice (white bars) and apoE4 mice (black bars) is depicted on the right. *p<0.05. (B) Confocal microscopy utilizing the apoE (green); neuronal marker NeuN (red); and GFAP (blue) antibodies. A representative section of the individual immunostainings and their superposition is presented. Colocalization of apoE with astrocytes is shown in light blue in the right panel (white arrowheads), whereas colocalization of apoE with neurons is shown in yellow (black arrowheads). Quantification of the relative percentage of apoE colocalized respectively with neurons and astrocytes (mean ± SEM; n=6 per group) in 1- and 4-month-old apoE3 mice (white bars) and apoE4 mice (black bars) was performed by computerized image analysis, as described in Materials and Methods, is presented on the right. Scale for left panel = 120 μ. Scale for the middle panel = 40 μ.