I/R induced time-dependent degeneration of inner retinal layers .Control and I/R mouse eyes were collected for histological assessment by H&E staining 3, 7, 14, 21 and 28 days after I/R injury with approximately 9-10 mice per time point. Whole retinal thickness (from NFL/RGCL to ONL) or sub-layer (IPL, INL and ONL) thickness at each time point was measured and statistically analyzed. Data represent mean ± standard error of percentages at each time point from both control and I/R eyes compared to the day 3-control eyes, which were set as 100%. For statistical analysis, two-way ANOVA (analysis of variance) was applied with the Bonfferoni post-hoc test. Asterisks indicate statistical differences (p < 0.05) between control and I/R eyes at each time point. (NFL/RGCL: nerve fiber layer/retinal ganglion cell layer, IPL: inner plexiform layer, INL: inner nuclear layer, ONL: outer nuclear layer).