Figure 4

Exercise induces antioxidant protection in brain capillaries of METH-exposed mice. Mice were exercised and exposed to METH as described in the legend to Figure 2. (A) Glutathione levels were determined in isolated brain capillaries by staining with monochlorobimane (MCB). The images (left panel) are representative data from three independent experiments and the quantified results (mean ± SEM) are depicted in the form of the bar graph (right panel). (B) The expression of phosphorylated Nrf-2 (p-Nrf-2) was determined in brain capillaries by immunoblotting and normalized to β-actin level. The blots are representative data from three independent experiments and the quantified results (mean ± SEM) are depicted in the form of bar graphs. *As compared to the respective controls at *p < 0.05 or ***p < 0.001. ^†Data in the exercised group are significantly different as compared to the respective treatment in the sedentary group at ^††p < 0.01 or ^†††p < 0.001.