Figure 2
From: Autophagy is involved in oligodendroglial precursor-mediated clearance of amyloid peptide
Aβ 42 uptake by NG2 cells. Primary NG2 cells (A) and NG2 cell line (B) were plated on coverslips for 18 hours and then incubated with HiLyte Fluor™ 555/488-labeled Aβ42 (2 μM) for 24 hours. Cells were then fixed and stained with anti-NG2 antibody. Cell nuclei were visualized by DAPI staining (blue). Aβ42 was engulfed by primary cultured NG2 cells and NG2 cells. Scale bars = 5 μm. C. Aβ42 uptake by NG2 cell line was shown by transmission electron microscopy (TEM). NG2 cell line were exposed to Aβ42 for 6 hours, stained with anti-Aβ42 IgG antibody followed by donkey anti-rabbit antibody conjugated to colloidal gold (18 nm particle), then processed by TEM. Internalized Aβ42 was demonstrated. The inset is an image of internalized Aβ42 with higher magnification. Scale bars = 0.2 μm.