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Figure 2 | Molecular Neurodegeneration

Figure 2

From: Autophagy is involved in oligodendroglial precursor-mediated clearance of amyloid peptide

Figure 2

42 uptake by NG2 cells. Primary NG2 cells (A) and NG2 cell line (B) were plated on coverslips for 18 hours and then incubated with HiLyte Fluor™ 555/488-labeled Aβ42 (2 μM) for 24 hours. Cells were then fixed and stained with anti-NG2 antibody. Cell nuclei were visualized by DAPI staining (blue). Aβ42 was engulfed by primary cultured NG2 cells and NG2 cells. Scale bars = 5 μm. C. Aβ42 uptake by NG2 cell line was shown by transmission electron microscopy (TEM). NG2 cell line were exposed to Aβ42 for 6 hours, stained with anti-Aβ42 IgG antibody followed by donkey anti-rabbit antibody conjugated to colloidal gold (18 nm particle), then processed by TEM. Internalized Aβ42 was demonstrated. The inset is an image of internalized Aβ42 with higher magnification. Scale bars = 0.2 μm.

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