Figure 3
From: Rapamycin increases survival in ALS mice lacking mature lymphocytes
Rapamycin does not affect survival of SOD1G93Amice, but increases survival of SOD1G93Amice lacking mature lymphocytes. SOD1G93A mice fed vehicle (n = 9) or rapamycin chow (n = 7) relative weight (A), onset-free survival (B) and disease duration (C). RAG1−/− SOD1G93A mice fed vehicle (n = 7) or rapamycin chow (n = 12) relative weight (E), onset-free survival (F) and disease duration (G). Survival analysis of SOD1G93A mice that were fed vehicle (152.6 ± 1.8 days, n = 17) or rapamycin chow (153.1 ± 2.5 days, n = 17) (D). Survival analysis of RAG−/− SOD1G93A mice fed with vehicle (148.6 ± 2.0 days, n = 23) or rapamycin chow (155.1 ± 1.8 days, n = 19, p = 0.04) (H). Western blot analysis of the levels of LC3-II (I), mTOR (J), phosphorylated mTOR (p-mTOR) (K), p62 (L) and NeuN (M) for end stage RAG1−/− SOD1G93A mice fed vehicle (n = 4) or rapamycin-containing chow (n = 4 and n = 5 for the analysis of p62 and NeuN). *p < 0.05, **p < 0.01.