Figure 2

Fusions of WT-mSod1 with YFP are fully soluble. WT-mSod1:YFP proteins were expressed in CHO cells for 24 h. Cells were fixed directly or treated with 0.1% Saponin prior fixation. Cells were immunostained with DAPI to allow visualization of cells nuclei. Note the camera exposure times used to capture YFP images were recorded as indicated in the figure. For this figure and the relevant figures that follow, the images shown are representative of 3 independent experiments.