Figure 7
From: Axonal BACE1 dynamics and targeting in hippocampal neurons: a role for Rab11 GTPase
Confocal analysis of internalized BACE1 localization in the soma of cultured hippocampal neurons. (A) Representative confocal images of the cell bodies of neurons co-transfected with BBS-BACE1-YFP and the indicated mCherry-tagged Rab11b WT, Rab11S25N, or empty vector constructs. Internalized BACE1 pool was labeled by AF647-BTX uptake, and the neurons were fixed following an acid wash to remove remaining surface staining. Neurons were then immunostained with markers of early endosomes (EEA1), lysosomes (LAMP1) and recycling endosomes (TfR), and analyzed by confocal microscopy. (B) The extent of colocalization between internalized BACE1 and endogenous organelle markers was quantified in the cell bodies of neurons transfected with the indicated constructs (n = 6-10 neurons each). Note that BACE1 is still able to reach TfR-positive recycling endosomes in the cell body after the overexpression of Rab11S25N.