Figure 8

Co-expression of the PolgA D257A mutation and APP / Ld transgene does not cause frank neuron loss. Representative coronal sections (30 μm) of brains from D257A; APP/Ld, D257A, APP/Ld, and wild type mice were selected from similar rostral-caudal positions, stained with hematoxylin, and images centering on cortical layer V were obtained at 100× magnification by light microscopy. Neuron density (number of neurons per mm²) was determined by counting the number of neurons in representative 100× fields of middle layers of dorsal cortex for the four genotypes of mice. Note that no significant differences in neuron densities were observed between the different genotypes (n = 5 mice/genotype; mean ± SEM; One-way ANOVA test followed by Newman–Keuls multiple-comparisons post hoc test).