Figure 2

6-OHDA rapidly decreases mitochondrial movement in non-DA axons. A) Axonal movement of mitochondria in control and 6-OHDA treated axons. Non-GFP positive axons (non-DA; Top panels) that were labeled with MitoDsRed2 (Middle panels) were selected for imaging 30 minutes after treatment with 6-OHDA. Resulting kymographs are shown below. For additional clarity tracks of moving particles are depicted in the bottom panels: blue lines denote anterograde movement and red lines indicate retrograde trafficking. Scale bar indicates 10 μm. Quantification of B) moving mitochondria in both anterograde and retrograde directions (n = 3–4 devices per group from with 3–5 axons analyzed per device) and C) mitochondrial speeds of motile mitochondria. The latter were calculated as described [10] (n = 90–120 mitochondria per group). In B and C, data are represented as mean ± SEM, *: indicate p < 0.05 versus control.