Figure 4

6-OHDA also decreases synaptic vesicle movement in DA axons. A) DA-GFP cultures (Top panels) in microdevices were transduced with Syn-Cer lentivirus (Middle panels) at DIV2. Vesicular movement was assessed on DIV12–13 before and after toxin treatment. Resulting kymographs are shown below. Because of the smaller size of vesicular particles and the relative “dimness” of the cerulean emission, tracks of moving particles are shown in bottom panels for clarity. Red represents retrograde movement whereas blue is anterograde trafficking. B) Quantification of moving vesicles was determined as described in Materials and Methods. Scale bar: 5 μm. Mean ± SEM, total of 8 (control) and 8 (6-OHDA-treated) axons from 5–7 devices per group. * indicates p < 0.05 versus control.