Figure 6
From: The Parkinsonian mimetic, 6-OHDA, impairs axonal transport in dopaminergic axons
Autophagy precedes cell death in midbrain neurons following 6-OHDA treatment. A) Autophagy was assessed by introducing a GFP-tagged LC3 expression clone at DIV6 and treating midbrain cultures 1 d later with 6-OHDA. LC3-positive puncta (arrows) were assessed by GFP fluorescence in representative neurons in control and after toxin treatment. B) The number of cells with at least three LC3-GFP puncta were counted and expressed as percentage of all neurons that were LC3-GFP positive, regardless of whether the LC3-GFP signal in these neurons was diffuse or punctated. Scale bar indicates 10 μm. Mean ± SEM from 3 independent experiments (n = 3–5 per group), *p < 0.05 versus control. C) Timeline of 6-OHDA induced events.