Figure 6

Abnormal cerebral blood flow increases amyloid pathology. A. and C. Representative images of CAA (A) and plaque (C) pathologies in anterior areas of control, sham-operated and transverse aortic constriction surgery (TAC)-operated APP DSL. TAC was performed on the left common carotid arteries of 6-month old APP DSL mice that terminated for analysis 3 months post-surgery. B. and D. Quantification of CAA (B) and plaque (D) load in left and right part of anterior (Ant.) or posterior (Post.) brain regions. Scale bars: 50 μm in A; 200 μm in C. N = 4 animals/group and 10 sections/animal. Coronal sections spanning from Bregma −2.58 ~ −0.58 mm were counted as anterior region;-0.94 ~ 3.64 mm were counted as posterior region. E-H, ELISA analysis of soluble (E and F) and insoluble (G and H) Aβ40 (E and G) and 42 (F and H) on anterior and posterior cortical areas in both left and right hemispheres. Each sample was measured in duplicates on ELISA plates. N = 3 animals/treatment. *P < 0.05, **P < 0.01, ***P < 0.001.