Table 4 RT qPCR results for two genes known to be upregulated in 5XFAD mice: comparison with the microarray results
qPCR | Microarray | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Age | Gene | Wt 1 | Wt 2 | Wt 3 | Tg 1 | Tg 2 | Tg 3 | Fold change Tg/Wt | Wt | Tg | Fold change Tg/Wt |
M1 | Aif1 | 1.3 | 0.9 | 0.8 | 1.3 | 1.3 | 0.9 | 1.2 | 2098.9 | 2010.3 | 1.0 |
Gfap | 1.5 | 0.9 | 0.7 | 1.3 | 1.4 | 0.8 | 1.2 | 6320.3 | 6827.6 | 1.1 | |
M4 | Aif1 | 1.1 | 1.1 | 0.9 | 2.8 | 1.7 | 1.3 | 3.1 | 2171.1 | 5335.4 | 2.5 |
Gfap | 0.9 | 1.3 | 0.9 | 5.5 | 6.1 | 5.1 | 5.5 | 5381.9 | 15329.9 | 2.9 | |
M6 | Aif1 | 0.9 | 1.0 | 1.2 | 2.8 | 1.7 | 1.3 | 1.9 | 2427.4 | 5530.1 | 2.3 |
Gfap | 1.1 | 1.0 | 0.9 | 3.9 | 5.6 | 4.2 | 4.5 | 5694.4 | 17759.7 | 3.1 | |
M9 | Aif1 | 1.4 | 0.9 | 0.8 | 2.6 | 2.0 | 2.3 | 2.3 | 2551.1 | 7422.3 | 2.9 |
Gfap | 1.5 | 0.8 | 0.9 | 5.0 | 7.0 | 5.9 | 5.9 | 5172.5 | 23062.3 | 4.5 | |