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Table 4 RT qPCR results for two genes known to be upregulated in 5XFAD mice: comparison with the microarray results

From: Temporal gene profiling of the 5XFAD transgenic mouse model highlights the importance of microglial activation in Alzheimer’s disease

  qPCR Microarray
Age Gene Wt 1 Wt 2 Wt 3 Tg 1 Tg 2 Tg 3 Fold change Tg/Wt Wt Tg Fold change Tg/Wt
M1 Aif1 1.3 0.9 0.8 1.3 1.3 0.9 1.2 2098.9 2010.3 1.0
Gfap 1.5 0.9 0.7 1.3 1.4 0.8 1.2 6320.3 6827.6 1.1
M4 Aif1 1.1 1.1 0.9 2.8 1.7 1.3 3.1 2171.1 5335.4 2.5
Gfap 0.9 1.3 0.9 5.5 6.1 5.1 5.5 5381.9 15329.9 2.9
M6 Aif1 0.9 1.0 1.2 2.8 1.7 1.3 1.9 2427.4 5530.1 2.3
Gfap 1.1 1.0 0.9 3.9 5.6 4.2 4.5 5694.4 17759.7 3.1
M9 Aif1 1.4 0.9 0.8 2.6 2.0 2.3 2.3 2551.1 7422.3 2.9
Gfap 1.5 0.8 0.9 5.0 7.0 5.9 5.9 5172.5 23062.3 4.5
  1. Before performing the microarray experiment, RNA from each wild type (Wt) and each transgenic (Tg) (n = 3 for each group) animal was tested independently by RT-qPCR for two genes known to be upregulated in 5XFAD mice: Aif1 and Gfap. This table compares results obtained by qPCR from each mouse with results obtained from the pooled RNA for all three animals in the microarray study. The qPCR and microarray gave similar fold changes, shown here in bold.