Figure 2

Aβ 1-42 induces intracellular full length tau (Tau441-YFP) aggregation in N2a cells. A) N2a cells were transfected with Tau441-YFP and treated with 200nM Aβ1-40 or Aβ1-42. 24 h later, cells were imaged directly under a confocal microscope. There was no difference in the expression of tau between the groups. B) Cells were extracted with 1% Triton during fixation, which left only insoluble tau in the cells. As shown in Figure B, Aβ1-42 treatment increased the level of insoluble full-length tau, but Aβ1-40 did not. C) Quantification of fluorescence intensity was done with Image J. Magnification: 63x, scale bar: 10 μm. Three individual experiments were done for each condition. *p <0.05.