Role of p53-IL-1β interaction on neuronal damage. A. The graphs show that time-dependent cell swelling was significantly more pronounced in slices incubated with IL-1β than in control conditions (p < 0.05) and that this effect was attenuated by CNQX (p > 0.05), a glutamate AMPA receptor antagonist, and PFT (p > 0.05). B. Examples of neuronal swelling induced by IL-1β in the presence or absence of PFT. Individual neurons were visualized in situ using a differential interference contrast optical system. C. p21 protein levels were determined in total protein extracts (300 μg) of mouse corticostriatal slices untreated or treated with IL-1β by Western blot analysis. Each lane represents a different animal and is composed by a triplicate experiment performed on the same animal. Densitometric analysis of each lane was calculated using Image J software and data were normalized to actin used as loading control. IL-1β significantly increases the expression of p21 protein. *means p < 0.05.