Figure 4

Immunofluorescence staining of neuronal and glial antigens following HIV-1 infection. Paraffin embedded 5 μM brain sections were immunostained with MAP2, SYN, NF, GFAP, MAG and Iba1 antibodies. (A) Representative images captured at 40× magnification from whisker barrel (WB), corpus collosum (CC) and Dentate gyrus, (DG) are shown for those markers significantly deregulated after HIV-1 infection. (B) Density of antigen expression quantified in different brain regions are shown for both HIV-infected and uninfected mice by multispectral imaging. Decreased expression of MAG and NF in WB, CC and DG regions of infected animals compared to uninfected controls. Significant increase in reactive microglia and astroglyosis (GFAP) was observed in DG. Oligodendrocyte associated myelin (MOG) was reduced both in WB and CC. Genu of corpus callosum from infected animals show decreased expression of MAG, MOG and NF in infected mice. Values are mean ± SEM and *denotes p < 0.05.