Figure 5

Membrane cholesterol increase in cultured cortical neurons leads to lower Aβ38 (38% less, p = 0.0017, unpaired Student’s t-test, n = 12 control and 10 cholesterol assays in three independent experiments) and higher Aβ42 secretion (24% more, p = 0.0334, unpaired Student’s t-test, n = 11 control and 10 cholesterol assays in three independent experiments), while Aβ40 stayed unchanged (less than 2% change, p = 0.6322, unpaired Student’s t-test, n = 16 control and 15 cholesterol assays in four independent experiments), as assessed by MesoScale Discovery dosage. ns stands for non-significant (p > 0.05), *for p < 0.05 and **for p < 0.01 in unpaired Student’s t-test.