Figure 6
From: Effects of the C57BL/6 strain background on tauopathy progression in the rTg4510 mouse model
Older rTg4510 B6 mice have increased soluble Tau5 and PHF1 levels compared to rTg4510 mice. (A-B) Western blot analyses of the soluble fractions of whole brain lysates from 6.5- and 10.5-month-old rTg4510B6 and rTg4510 mice. (A) Representative western blots of soluble human tau, human and mouse tau, and phosphorylated tau and with a NT littermate shown as a negative control. (B) Densitometric quantification of E1 (human tau), Tau5 (human and mouse tau), CP13 (pS202 tau) and PHF1 (pS396/404 tau) normalized to GAPDH. rTg4510B6 mice had equivalent levels of soluble CP13 and human tau, and increased soluble total tau (human and moues) and PHF1 tau compared to rTg4510 mice on the original strain background. Each dot represents an individual mouse with the mean indicated by the black line. n = 3-4 per cohort. **p < 0.01 [two-way ANOVA (strain x age): main effect of strain indicated].