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Figure 6 | Molecular Neurodegeneration

Figure 6

From: Posttranslational modification and mutation of histidine 50 trigger alpha synuclein aggregation and toxicity

Figure 6

Fibrillization of WT and H50 mutant aSyn. A) EM analysis of fibrils derived from recombinant WT and H50Q/R aSyn agitated for 7 days. B) Recombinant WT and H50Q/R aSyn were agitated for 7 days with (+NaCl) and without (-NaCl) 160 mM NaCl. The formation of aSyn fibrils was assessed by ThT assay. ThT intensity was normalized to non-agitated, monomeric WT aSyn (M, dashed line). H50Q aSyn mutation significantly increases the formation of aSyn fibrils with and without NaCl. Significance was determined by one way ANOVA Tukey’s Multiple Comparison Tests. n = 6 (-NaCl), n = 8 (+NaCl). Error bars = SEM.

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Molecular Neurodegeneration

ISSN: 1750-1326