Figure 5

The N-Cadherin mediated degradation of CBP was induced by Aβ. (A) CBP degradation was mediated by N-Cadherin. HT22 cells transiently transfected with the N-Cadherin construct. CBP expression was significantly decreased by N-Cadherin transfection. (B) N-Cadherin CTF expression was increased by Aβ. HT22 cells were treated with Aβ (0 or 2 μM) for 24 h. Western blot analysis revealed that N-Cadherin CTF expression was elevated by Aβ in a dose dependent manner. (C) γ-secretase inhibitor treatment increased CBP expression. HT22 cells were treated with L-685,458. Western blot analysis showed that CBP expression was increased by L-685,458 in a dose dependent manner. (D) γ-secretase inhibitor treatment rescued Aβ-induced CBP degradation. HT22 cells were incubated with Aβ only, or Aβ combined with L-685,458, for 24 h. Western blot analysis revealed that Aβ-induced CBP degradation is ameliorated by L-685,458. (E) Densitometric analysis of CBP protein from three independent experiments. CBP was normalized to Lamin A. ^** P < 0.01, ^## P < 0.01.