Figure 9

Immunization with AFF 1 increases microglial α-syn clearance in MBP-α-syn tg mice. (A) Double immunostaining for the microglial marker Iba1 (red) and α-syn (green) in vehicle- and AFF 1-immunized MBP-α-syn tg mice. The location of Iba1-positive cells is denoted by arrows and the location of α-syn-positive cells by asterisks. Cell nuclei were stained with DAPI (blue). Scale bar = 10 μm. (B) Quantification of the percentage of colocalization between Iba1 and α-syn (C) Double immunostaining for the oligodendroglial marker p25 (red) and α-syn (green) in vehicle- and AFF 1-immunized MBP-α-syn tg mice. The location of p25-positive cells is denoted by arrows. Cell nuclei were stained with DAPI (blue). Scale bar = 10 μm. (D) Quantification of the percentage of colocalization between p25 and α-syn (E) Double immunostaining for the astroglial marker S100 (red) and α-syn (green) in vehicle- and AFF 1-immunized MBP-α-syn tg mice. The location of S100-positive cells is denoted by arrows and the location of α-syn-positive cells by asterisks. Cell nuclei were stained with DAPI (blue). Scale bar = 10 μm. (F) Quantification of the percentage of colocalization between S100 and α-syn (G) Double immunostaining for the neuronal marker NeuN (red) and α-syn (green) in vehicle- and AFF 1-immunized MBP-α-syn tg mice. Cell nuclei were stained with DAPI (blue). Scale bar = 5 μm. (H) Quantification of the percentage of colocalization between NeuN and α-syn. Results are expressed as average ± SEM. (*) p < 0.05 when comparing vehicle-treated tg animals with AFF 1-treated tg animals by Student’s t-test. n = 10 animals per group.