Figure 6
From: Analysis of tau post-translational modifications in rTg4510 mice, a model of tau pathology
Age-dependent change of ubiquitination and nitration of tau in the brain insoluble fraction of rTg4510 mice. Post-translational modifications were measured using AlphaLISA immunoassays with 1 μg of protein from the brain insoluble fraction of rTg4510 mice. A. Age-dependent change of tau ubiquitination. Ub-tau was detected using biotin HT-7 for capture and an anti-ubiquitin antibody for detection. B. Age-dependent change of Tyr29 nitration of tau. nY29 tau was detected using biotin HT-7 for capture and an anti-nY29 antibody for detection. C. and D. Correlation of Ub-tau (C) and nY29 tau (D) with PHF6 tau. Correlation analysis was done using Prism 5.0 software.