Figure 4

Wild-type and mutant HTT mRNA levels in juvenile HD brain. Wild-type and mutant HTT mRNA PCR products were separated by gel electrophoresis. (A) RT-PCR products from brain tissue derived from two juvenile HD patients (HD192 and HC104). CAG repeat sizes for the wild-type and mutant alleles are indicated below each lane. gDNA was used to control for differences in PCR amplification between the wild-type and mutant product due to the CAG repeat expansion. (B) Whisker box plot comparing wild-type and mutant HTT mRNA expression levels, relative to gDNA. Pair wise differences were evaluated using linear mixed model, n = 4.