Fig. 3
From: Mutations in LRRK2 potentiate age-related impairment of autophagic flux
LRRK2 modifies autophagic flux. a. Fluorescence of the dat-1::lgg-1::mCherry reporter in nematode lines co-expressing snb-1::LRRK2. Expressing WT and kinase dead (KD) LRRK2 greatly increases autophagic flux, while G2019S or R1441C expression decreased autophagic flux, causing a corresponding increase in fluorescence. Scale bar = 20 μm. b. Quantification of total fluorescence from four CEP neurons and lateral part of nerve ring for each of the nematode lines (Ctrl, lgg-1 and lgg-1/LRRK2 (WT, KD, G2019S and R1441C) at day 5 of life. c. Immunoblot showing total lgg1::mCherry protein derivatives from the age synchronized whole animals at day 5 of age. The arrow points to lgg1::mCherry. d. Total mCherry fluorescence from four CEP neurons and lateral part of nerve ring captured in age synchronized nematode lines (Ctrl, lgg-1 and lgg-1/LRRK2 (WT, G2019S anR1441C) over much of the lifespan. N = 40 animals/condition, **P < 0.001 and *P < 0.05 compared to lgg-1::LRRK2 (WT). lgg-1::LRRK2 (G2019S) differed from lgg-1 only at day 13 (P < 0.05). lgg-1::LRRK2 (R1441C or Δlrk-1) differed from lgg-1::LRRK2(WT) at time points of 7 days or greater