Fig. 4
From: Reduced secreted clusterin as a mechanism for Alzheimer-associated CLU mutations
Visualization and quantification of CLU secretion for different CLU coding mutations in HEK Flp-In cells. a Immunoblot visualization of CLU Flp-In cells, showing lower sCLU levels in cell media (CM) and increased expression of full length (FL) CLU in cellular lysates (CL) for p.R338W and p.I360N. Alpha-tubulin staining is incorporated as an equal loading control. b ELISA quantification of CLU in HEK Flp-In CM and CL, presented as relative proportions of total CLU in CM and CL, displays a significant ratio decrease for p.R338W and p.I360N (*p < 1×10−4). N corresponds to the number of independent experiments. c Quantification of western blot band intensities in CM and CL of HEK Flp-In cells, presented as relative proportions of total CLU in CM and CL, with a significantly decreased CLU ratio of CM/CL for p.R338W and p.I360N (*p < 1×10−4). N corresponds to the number of independent experiments. d Western blot CLU intensities in CM (blue) and CL (red) normalized against α-tubulin and logarithmically rescaled against wt. a-d Mutant p.I303NfsX13 was not included in these experiments because lack of the ß-chain interferes with antibody binding