Fig. 1
From: Synaptotagmins interact with APP and promote Aβ generation
Mass spectrometry-based identification of novel APP-interacting proteins. a Colloidal Blue-stained gel of APP-interacting proteins from mouse forebrain extracts. The proteins were pulled down with GST-tagged APP-ectodomain regions. Red asterisks denote purified GST-tagged APP-ectodomain fragments while arrows point to the bands that were excised and subjected to MS-based analysis for protein identification. Arrowheads denote bands containing Syt peptides. b MS-based confirmation of previously identified APP ectodomain-interacting proteins. c MS-based identification of Syt-1, Syt-2 and Syt-9 peptides. Peptides from all three Syts were pulled down by GST-APP and GST-E1 + KPI. Other GST-tagged APP fragments did not pull down Syt peptides (not shown)