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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Studies of lipopolysaccharide effects on the induction of α-synuclein pathology by exogenous fibrils in transgenic mice

Fig. 1

Assessment of endotoxin/LPS contamination in αS protein preparations. a Schematics showing the charged regions and hydrophobic regions of αS and LPS. NAC = “non-amyloid-β component of the amyloid plaque” hydrophobic region of αS. (b and c) Average quantification of endotoxin contaminants in αS protein preparations. Results are also shown for samples that were further purified using High S resin (cation exchanged). (b) and (c) illustrate contamination in the same sample set, using the Pierce LAL assay and the InvivoGen HEK-Blue-hTLR4 cell culture system respectively. The hTLR4 assay shows an expanded standard curve. 1 μM αS = 0.0146 mg/ml. White bars represent the endotoxin standard and black bars represent test samples. Error bars represent the standard error of the mean. AU = absorbance units

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Molecular Neurodegeneration

ISSN: 1750-1326