Fig. 2

αS pathology in M83 αS Tg mice following injection of αS or cation exchanged αS fibrils. a Representative immunohistochemical images of cortical, hippocampal, brainstem, and spinal cord sections of hemizygous M83 αS Tg mice stereotaxically injected in the hippocampus with αS or LPS purified (cation exchanged) αS. Antibodies to αS (Syn506), to αS phosphorylated at Ser129 (pSer129/81A), and a general inclusion marker (p62) were used. Scale bar = 50 μm. b Schematic representation of the distribution of αS inclusion pathology in the neuroaxis of hemizygous M83 αS Tg mice injected with αS or cation exchanged αS fibrils. Red dots indicate the sites and amounts of αS pathology. Blue arrows indicate the site of injection. c Quantification of Syn506 staining in the hippocampus of mice injected with αS or cation exchanged αS fibrils. The p-value was calculated using a two-tailed t-test. Error bars represent the standard error of the mean