Fig. 4
From: Conditional inactivation of Akt three isoforms causes tau hyperphosphorylation in the brain
Increased levels for phosphorylated PKA substrates in Akt cTKO mice. a Western blotting for phosphorylated PKA substrates using cortical samples of Akt cTKO mice. Levels for several phosphorylated PKA substrates with molecular weights at about 100 kDa, 60 kDa and 40 kDa were increased. b Western analyses on VASP and PKA subunits. P-VASPSer157 levels in Akt cTKO mice were highly increased but total VASP levels were not changed. Relative levels for PKA regulatory subunits, 1α and 1β, were not changed. GAPDH served as the loading control. c Western blotting on p-tauSer214 and p-tauSer356. Ratios of p-tau to total tau were significantly increased in Akt cTKO mice, as compared to control animals (*, p < 0.05)