Fig. 5

MiR-9 induced the increased of total Nav1.1 and Nav1.2 in primary cultured neonatal rat neurons (NRNs). a,b Effects of miR-9 on total protein levels of endogenous Nav1.1 (a), Nav1.2 (b), in NRNs, using western blot analysis and . Cells were transfected with miR-9, AMO-9, miR-9 + AMO-9, or NC. mean ± s.e.m from 3 batches of cells for each group. *P < 0.05 vs NC; ^# P < 0.05 vs miR-9. c-d,Representative confocal microscope images showing primary culture hippocampus neuron stained for Tubulin (green, upper), Nav1.1 (C)/Nav1.2 (D) (red, middle). A merged image depicting double positivity (yellow) is shown on the bottom after transfection with miR-9 mimics or/and AMO-miR-9, negative control