Fig. 7

STIM2 overexpression protects mushroom spines from amyloid toxicity in vivo. a The spine shape of CA1 hippocampal neurons from 3.5 months old line M mice injected with AAV1-mSTIM2 (S2), Aβ42, control mice (Ctrl), and Aβ42 together with AAV1-mSTIM2 (S2) was visualized by two-photon imaging. Scale bar corresponds to 5 μm. Mushroom spines are indicated with an arrow. b, c Total spine density and percentages of mushroom spines (MS) in neurons from CA1 hippocampal area of mice injected with AAV1-mSTIM2 (S2), control mice, mice injected with Aβ42 and mice injected with Aβ42 together with AAV1-mSTIM2 (S2). Graph represents the data of three independent experiments. For spine quantification n = 21–30 neurons were analyzed. Values are shown as mean ± SEM. ***: p < 0.0005 by t-test