Fig. 6
From: Human neural stem cells alleviate Alzheimer-like pathology in a mouse model
hNSCs reduce the expression of pro-inflammatory mediators. a Comparison of the expression of Il1b, Il6, Tnfa, and iNOS mRNA levels in the brains of hNSC-injected (NSC, n = 7) and vehicle-injected (Veh, n = 6) NSE/APPsw transgenic mice. b and d The expression of Il1b, Il6, and Tnfa in LPS-stimulated BV2 microglial cells co-cultured with hNSCs (BV2 + NSC) compared with that in single cultures of LPS-stimulated BV2 cells (BV2) on Transwell permeable supports (n = 3 per group; b). The hNSCs co-cultured with BV2 cells (NSC + BV2) express TGFB1, IL4, and IL13 (d). c and e In mixed cultures, the change of Il1b, Il6, Tnfa, and iNOS expression in LPS-stimulated BV2 cells (BV2 + NSC) co-cultured with hNSCs compared with that in single cultures of LPS-stimulated BV2 cells (BV2; n = 3 per group; c). The hNSCs co-cultured with BV2 cells (NSC + BV2) express TGFB1, IL4, IL13, CX3CL1, CD47, and CD200 (e). f The relative expression of mRNA for Il1b, Il6, Tnfa, and iNOS in Tgfbr2-, Sirpa-, and Cd200r1-knockdown BV2 cells co-cultured with hNSCs compared with that in non-functioning negative siRNA (siNega)-transfected BV2 cells in mixed co-culture in the presence of LPS (n = 3 per group). The number of mice (n) in A is indicated. The number of experiments (n) in (b, c), and (f) is indicated. All data represent mean ± SEM. Error bars indicate ± SEM. *p < 0.05, **p < 0.01